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1.
Rev. chil. tecnol. méd ; 19: 817-20, 1999.
Article in Spanish | LILACS | ID: lil-295209

ABSTRACT

Enzyme immunoelectrotransfer blot (EITB) has received increasing attention as a confirmative technique for the serodiagnosis of different infectous diseases. Also, EITB has bee used for identifying immunodominant polipeptides as potential antigenic components of parasite's extracts. In this study we used EITB to detect IgM specific antibodies in sera of patients with parasitological confirmed fascioliasis. Our prelininary results show that IgM reactivity was observed in 9 of the 17 sera of infected patients. The sensitivity of EITB was 52.9 percent, with at least 10 IgM immunoreactive bands. Futher studies are needed to define the specificity of the bands observed and to detect IgM immunoreactive polipeptides of Fasciola hepatica in patients with pre-patent fascioliasis. In the parasitological confirmed human infections by F. hepatica the sensitivity of IgM EITB was low and similar to those observed by using IgM ELISA


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Fascioliasis/immunology , Immunoblotting , Antibodies, Helminth , Fasciola hepatica/isolation & purification , Fascioliasis/diagnosis , Immunoglobulin M/immunology , Immunoenzyme Techniques/methods , Serologic Tests/methods
2.
Arch. argent. pediatr ; 84(6): 360-2, 1986.
Article in Spanish | LILACS | ID: lil-45726

ABSTRACT

Se presentan los hallazgos clínicos, de laboratorio y la evolución de dos niños con síndrome nefrótico neonatal asociado a sífilis congénita. La VDRL fue positiva en los dos al igual que en sus madres, las cuales no habían recibido tratamiento específico. Ambos presentaron proteinuria masiva, hipercolesterolemia e hipoalbuminemia. La disminución del complemento sérico C3 sugiere una patogenia inmunológica de la enfermedad. Con el tratamiento específico instituido, los pacientes se recuperaron del síndrome nefrótico. Uno falleció algunos meses después por una sepsis a gramnegativo y el otro fue controlado ambulatoriamente, hallándose clínica y humoralmente normal


Subject(s)
Infant, Newborn , Humans , Nephrotic Syndrome , Syphilis, Congenital
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